A group of four men with pale skin, shown from the waist up, almost fill this square painting. Light pours onto the scene from the upper left and falls across the upturned face of the bearded, bare-chested man, Saint Bartholomew, who is situated with his back facing us and his arms spread wide. In the top right corner, his right wrist is lashed to a pole that extends off the top edge of the painting. His other arm is bent at the elbow near the lower left corner, and he holds that hand palm up. His profile, facing our left, is bright against the shadowed background. His mouth is slightly agape, and his eyebrows are raised as he looks up toward the light that bathes his lithe, muscular body. His long beard is streaked with gray, and he has receding black hair. A second, broad-shouldered man, draped in a brown cloak, stands facing us behind and just to our left of Saint Bartholomew. The younger man holds a knife in one hand resting against a black, rod-like sharpener held in the other. He turns his head to gaze at Saint Bartholomew, his eyes deep in shadow. He also has a furrowed brow and a craggy, ruddy complexion. Two more men stand behind him, to our left, filling the left side of the painting. The man on the far left has smooth skin, a trimmed beard, and short brown hair. He stands in profile looking to our right and wears a cranberry-red cloak. Barely visible beyond him, to our right, is a man wearing a gray-green hood hiding half his face. His features are loosely painted and faint, but he also looks up, either toward the young man or the light from above. All four are surrounded by deep shadows against a brown background.